Project Number: 5030-32000-226-17-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jun 1, 2020
End Date: Dec 31, 2020
The objective of this proposal is to evaluate the ability of non-encapsulated and nanoparticle-encapsulated NK2A peptide to (1) kill MDR Salmonella isolates in host cells, and (2) prevent biofilm formation and/or disrupt pre-formed MDR Salmonella biofilms.
We will collaborate with the Cooperator to encapsulate the NK2A peptide into biodegradable nanoparticles for improved delivery, stability, and bacterial killing. Nanoparticle encapsulation and NK2A release will be optimized, host cell cytotoxicity will be analyzed using various cell lines, and intracellular killing of diverse MDR Salmonella will be evaluated. Anti-biofilm activity of NK2A against MDR Salmonella will be assessed using bacterial viability assays and electron microscopy.