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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Research Project #438250

Research Project: Temporal Control of Immunity to Powdery Mildew in Barley

Location: Corn Insects and Crop Genetics Research

Project Number: 5030-21000-067-03-I
Project Type: Interagency Reimbursable Agreement

Start Date: Jun 1, 2020
End Date: May 31, 2023

Objective:
Determine regulators of immunity to powdery mildew in barley and validate role of cis-regulatory element (CRM) - regulated genes

Approach:
SPECIFIC AIM 1: Use genome-wide promoter-motif enrichment strategies to uncover master regulators of immunity With the advent of a new high-quality, megabase resolution barley assembly, our aim is to leverage our accumulated expression data to facilitate identification of cis-regulatory elements (CRM), and associated expression cassettes associated with immunity. SPECIFIC AIM 2: Functional validation of cis-regulatory element (CRM)-regulated genes and associated targets via reverse genetic analysis and high-throughput phenotyping