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ARS Home » Southeast Area » Mississippi State, Mississippi » Crop Science Research Laboratory » Genetics and Sustainable Agriculture Research » Research » Research Project #438034

Research Project: Evaluation of Tri-species Chromosome Substitution Lines (CS) with Novel Alleles for Valuable Traits (CI Project# 19-115)

Location: Genetics and Sustainable Agriculture Research

Project Number: 6064-21000-016-17-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Jan 1, 2020
End Date: Dec 31, 2020

Objective:
Create and evaluate a unique set of germplasm by targeted introgression of desirable genes for fiber quality, oil and protein percentage of the cottonseeds from three tetraploid species including wild and unadapted types using about 53 different chromosome substitution (CS) lines from G, barbadense, G mustelinum and G. tomentosum.

Approach:
The recurrent parent TM-1 and the selected 53 CS lines from the crosses of the CS lines from G. mustelinum (CS-M), G. barbadense (CS-B), G. tomentosum (CS-T) and TM-1 will be used for field evaluation of agronomic and fiber traits at two locations (USDA/ARS, Mississippi State in replicated trial plots in summer of 2020). A 25-boll hand-harvested seedcotton sample will be collected from each plot prior to machine picking. These samples will be weighed to determine boll size and ginned on a laboratory 10-saw gin to determine lint percentages and to provide lint samples for fiber analyses. Fiber samples will be sent to Cotton Incorporated for HVI quality determination. Yield will also be determined. Direct comparisons among the lines and TM-1 will be used to detect effects of the substituted chromosomme on important characteristics including fiber, oil and protein percentage of the seeds.