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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #438023

Research Project: Development of Gene-editing Technologies in Livestock to Address Agriculturally Relevant Problems

Location: Plant Genetics Research

Project Number: 5070-31000-001-000-D
Project Type: In-House Appropriated

Start Date: Apr 6, 2020
End Date: Sep 30, 2022

Objective:
Objective 1: Using gene editing techniques, systematically alter DNA sequence predicted to cause beneficial changes in pig production and test these changes in vitro and in vivo where warranted. Objective 2: Comprehensively test effects of sequence modifications for on and off target effects. Objective 3: Develop efficient methods for gene modification of poultry.

Approach:
Using gene editing techniques, we will systematically alter DNA sequence predicted to cause beneficial changes in pig production and test these changes in vitro and in vivo where warranted. In addition, we will comprehensively test effects of sequence modifications for on and off target effects. Gene editing and other technologies will be used to systematically alter DNA sequence in ways that were predicted to improve traits to (1) determine whether the sequence variation is causal for the change in the trait, and (2) determine any other changes in traits that might simultaneously occur through pleiotropy effects. The research will focus on pigs, and the goal will be to develop pigs with improved traits of interest to the swine industry, without having deleterious effects on swine production. Simultaneously, we will work toward understanding relationships between genes and various physiological functions within pigs. This information may also be valuable to both humans and other livestock species.