Location: Cereal Disease Lab
Project Number: 5062-21220-023-027-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Apr 1, 2020
End Date: Mar 31, 2021
Objective:
the objectives of this project are to: 1) Sequence additional 168 mutant durum wheat lines using Cooperator’s promoter capture platform to expand the sequenced TILLING population; 2) Characterize the chemically induced sequence variation in the promoters of this population using the available bioinformatics pipeline; 3) Screen the lines for changes in disease resistance loci; 4) Add the new mutations to current mutant databases; 5) Identify candidate disease resistance gene(s) for further molecular analysis.
Approach:
The DNA from EMS mutants of a durum wheat cultivar will be isolated and promoters for about 82,000 genes will be captured using Cooperator’s standard protocol. These promoters will then be sequenced and compared with that of parental lines to determine the mutations in each specific lines.
These data will be uploaded into a database for easy search of mutations in specific genes. Additionally, the seeds of these lines will be made available for screening against known rust or Fusarium isolates. These genes will then be identified by looking at the mutations in that specific line. Further analysis will be performed for identification of candidate genes and characterization of the molecular pathways.
