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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #437800

Research Project: Increasing Genetic Diversity, Yield, and Protein of US Commercial Soybean Germplasm

Location: Plant Genetics Research

Project Number: 5070-21000-040-37-A
Project Type: Cooperative Agreement

Start Date: Oct 1, 2019
End Date: Jul 31, 2021

Objective:
In this project, we are using both elite and exotic germplasm sources to enhance yield and protein concentration in soybean. Members of our team have been involved in long-term research to increase soybean seed protein concentration, including mapping regions of the soybean genome that are associated with yield and seed composition traits, identifying candidate genes that are responsible for the observed trait modifications, and characterizing amino acid and protein subunit composition of soybean seeds.

Approach:
Characterize protein composition,and amino acid profile of seeds from our experimental extreme high-protein lines by HPLC, 2-D gel electrophoresis and mass spectrometry. Transcriptome profiling of selected high-protein lines and parents from both the UP2 and UP3 populations using Illumina high-throughput transcriptome sequencing (RNA-Seq) will also be performed.