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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Research Project #437792

Research Project: Developing the Perfect Molecular Markers and New Germplasm for Rapid Incorporation of Resistance to Soil Borne Pathogens in Soybean

Location: Corn Insects and Crop Genetics Research

Project Number: 5030-21220-006-51-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Jan 1, 2020
End Date: Feb 28, 2021

Objective:
1. Use RNA-seq to characterize lines containing mapped resistance traits to characterize resistance loci, characterize defense networks and generate candidate polymorphisms for marker development. 2. Characterize candidate resistance genes using virus induced gene silencing and or CRISPR/cas9 gene editing technology. 3. Improve protocols and methods for virus induced gene silencing to facilitate disease phenotyping.

Approach:
1. Fine map soybean germplasm with resistance to P. sojae, Pythium spp. and F. graminearum. 2. Conduct RNA-seq to compare gene expression in response to pathogen infection in resistant (mapped) and susceptible germplasm. 3. Use Virus Induced Gene Silencing (VIGS) to assay the function of candidate resistance genes. Use gene editing technology to assay the function of candidate resistance genes. 4. Release new germplasm with enhanced resistance to seedling diseases