Project Number: 6040-32000-009-16-I
Project Type: Interagency Reimbursable Agreement
Start Date: Oct 1, 2019
End Date: Sep 30, 2020
The Fellow wishes to accomplish the following: 1) Learn advanced skills in Antimicrobial Resistant (AMR) and related field under one health framework; 2) Fulfill AMR work that have not been fundable in previously proposed research; 3) Learn skills to improve the quality of his work and future activities on AMR in foodborne bacteria; 4) Complete research to fill crucial data gaps on AMR in Cambodia; 5) Actively fulfill the National Action Plan on AMR in Cambodia, which is completing in Jan 2019; and 6) Build a better connection between Cambodia and the USA through working with his mentor during the fellowship and his/her visit to Cambodia.
To achieve the Fellow’s goals the research plan will consist of three parts: 1) Basic bacteriology, culturing of Salmonella from poultry, and antimicrobial susceptibility testing (AST); 2) Current methods of PCR identification of AR genes, especially MCR genes encoding colistin resistance in Salmonella, DNA sequence analysis of those AR genes to investigate the phylogeny of MCR genes in Cambodian Salmonella; and 3) Advanced technique of whole genome sequence (WGS) analysis of selected Salmonella isolates from Cambodia. These three objectives start with mastering basic methods in AST and bacteriology, then move to the current standard of PCR and gene sequencing to analyze Antimicrobial Resistant (AMR), then end with the most advanced technique widely available, WGS. Therefore, this plan will not only give the fellow his desired training and help fill data gaps on AMR and MCR genetics of Salmonella poultry isolates in Cambodia, but also prepare the Fellow for the future of AMR research with WGS analysis. Completing this work will also establish a collaboration between the ARS mentor and the Cambodian Fellow. This relationship is expected to be long lasting, result in published scientific findings, and build strong ties between USDA-ARS and Cambodian food safety and agricultural programs. To aid in facilitating this goal, the mentor will visit the Cambodian fellow's agency, meet with Cambodian agricultural administration, and give several lectures on their collaborative work.