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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Research Project #437653

Research Project: Generation of Zoonotic Influenza Resistant Recombinant Pigs via Site-Directed Technology

Location: Virus and Prion Research

Project Number: 5030-32000-120-029-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2019
End Date: Dec 31, 2021

Objective:
The objective of this proposal is to develop a recombinant pig via site-directed genetic modifications that are resistant to zoonotic influenza A viruses (IAV) and which will not transmit swine influenza.

Approach:
Genome editing tools and innovative influenza resistance tools will be employed to engineer influenza resistance in pigs. Aim-1 will assemble and test composite vectors for targeting IAV replication, assembly, and dissemination of swine adapted IAV strains ex vivo. Aim-2 will create genome edited pigs designed to be resistant to IAV infection and transmission. Aim-3 will challenge the recombinant pigs with swine adapted IAV subtypes and assess viral production and transmission rates.