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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Research Project #437614

Research Project: Understanding the Role of Lettuce Leaf Lipid Composition in Salinity Tolerance

Location: Crop Improvement and Protection Research

Project Number: 2038-21530-002-026-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Nov 1, 2019
End Date: Apr 30, 2022

Objective:
We aim to perform deep lipidomic studies to analyze phospholipid and fatty acid composition in leaves of 10 sensitive and tolerant lettuce varieties selected from our previous study. In addition, we will screen lettuce germplasm and analyze fatty acid (including omega-3) composition in leaves, correlating with salinity tolerance and good seed germination under high salinity.

Approach:
We will conduct experiments aimed at method development and workflow optimization for lipid extraction and lipidomics analysis. We will perform extraction and analysis of lipids from plant tissue for phospholipid composition and fatty acid composition from 10 genotypes selected previously for proteomics, transcriptomics etc. studies will be performed. Lipids will then be sent to the Kansas Lipidomics Research Center for lipidomics analysis. Screening of lettuce germplasm for fatty acid composition will also be carried out. We will combine results from lipidomics with previous proteomics, transcriptomics, glycomics and physiology results and perform detailed statistical analyses to identify factors that are important for salinity tolerance in lettuce. Based on these results, we will select and plant salinity tolerant varieties to cross. Seeds from crosses will be screened for tolerance to salinity in growth chamber and field.