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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #437448

Research Project: Improving on Perfection; Gene Editing to Reduce or Eliminate Anti-Nutritional Factors in Soybean Seeds that have been Shown to Reduce Economic Value

Location: Plant Genetics Research

Project Number: 5070-21000-040-29-A
Project Type: Cooperative Agreement

Start Date: Oct 1, 2019
End Date: Aug 31, 2021

Objective:
Develop soybean lines that have reduced anti-nutritional factors in seeds. The two major proteinaceous anti-nutritional factors (ANFs) in soybean meal are the Kunitz- trypsin inhibitors (KTi) and Bowman-Birk inhibitors (BBi). We will develop soybean germplasm that will benefit the end users by drastically lowering or eliminating two major proteinaceous anti-nutritional factors (ANFs) in soybean meal, Kunitz- trypsin inhibitors (KTi) and Bowman-Birk inhibitors (BBi).

Approach:
Previous research by our group identified a combination of two mutant genes that results in significantly lower trypsin inhibitor activity than has previously been reported by any soybean group. We will use gene editing, via the CRISPR CAS9 system, in order to induce deletion mutations which will inactivate all seed expressed BBi genes in a high-yielding cultivar of soybean.