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ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Research Project #437411

Research Project: Genomics-Enabled Plant Biology for Determination of Gene Function

Location: Crop Production and Pest Control Research

Project Number: 5020-21000-008-015-I
Project Type: Interagency Reimbursable Agreement

Start Date: Oct 1, 2019
End Date: Sep 14, 2023

Objective:
Characterize the role played by key transcription factors (TF) in regulating seed oil traits of biofuel interest.

Approach:
From the large public RNA-Seq data set in Arabidopsis we first identified all seed expression sets using an automated classifier, and then inferred a seed-specific gene regulatory network. The transcription factors (TFs) in the network were ranked by their relative influence on the lipid biosynthesis genes. Four well-known regulators of seed lipid biosynthesis were discovered ab initio in this network. In the proposed project we will build on this approach by adding a TF redundancy index to prioritize non-redundant TFs and then validate their influence on seed lipid biosynthesis via phenotypic assays and ChIP-Seq assay to find their targets. For TFs that are candidates for regulating oil biosynthesis, knock-outs and overexpressers will be constructed and seed oil quantity and quality will be measured in these lines.