Location: Plant Genetics Research
Project Number: 5070-21000-043-009-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Oct 1, 2019
End Date: Sep 30, 2021
The over-arching objective of this research is to enhance the seed quality in soybeans by limiting production of carbohydrates that are not digestible and increasing the final concentration of oil.
Isotopic labeling pulse-chase experiments will be used to study the flow of carbon during seed desiccation. Transcriptomics provided by collaborators will complement the labeling experiments. We will focus on central carbon metabolism and those steps associated with remobilization of seed lipids that we hypothesize may account for changes in carbohydrate levels. Labeling in the form of stable isotope (13C) experiments will be used to judge metabolic activity with quantitative analyses by liquid chromatography linked to tandem mass spectrometers. The project will result in metabolic flux maps of soybean seeds during late stages of seed development and highlight pathway operation to guide metabolic engineering efforts.