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ARS Home » Southeast Area » Mississippi State, Mississippi » Poultry Research » Research » Research Project #437284

Research Project: Identification of the Molecular Determinants of Pathogenesis, Strain Variation, and Tissue Tropism of Different E. coli Strains

Location: Poultry Research

Project Number: 6064-13000-013-009-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 31, 2019
End Date: Jul 30, 2024

Objective:
To use proteomics, genomics, and systems biology approaches to identify molecular determinants of pathogenesis, strain variation, and tissue tropism of different Escherichia (E.) coli strains.

Approach:
Escherichia (E.) coli isolates isolated from poultry demonstrating clinical disease (e.g. colibacillosis) will be collected from poultry-rich geographical areas. Genomic DNA will be isolated and 16S rDNA sequencing will be used to confirm identification. Subsequently, the strains will be differentiated via RFLP and/or MALDI-TOF Mass Spectrometry. The whole genome of the individual isolates will then by sequenced and the genomes assembled. Comparative genomic sequence analysis of the E. coli strains will be used to identify specific genes and proteins that are recognizable by the host immune system.