Location: Crop Genetics and Breeding Research
Project Number: 6048-21000-031-001-I
Project Type: Interagency Reimbursable Agreement
Start Date: Oct 1, 2019
End Date: Aug 31, 2024
Objective:
1) Gain a deeper understanding of the extent of variation that we have observed in sterile diploid hybrids that is then retained upon chromosome doubling to the tetraploid state and inherited by tetraploid offspring.
2) More narrowly define genetic markers for resistance to tomato spotted wilt virus from Arachis diogoi that can contribute to peanut cultivar improvement.
3) Use 16 diverse peanut genotypes to develop a multi-parent advanced generation intercross (MAGIC) population encompassing a broad spectrum of disease and pest resistances, as well as quality traits, to increase resolution of genetic markers.
4) Use the outputs from objectives 2 and 3 to develop marker panels that can be used by breeders for marker assisted selection.
Approach:
1) Interspecific hybrids will be made and genetically analyzed to search for gene bias that might generate genetic diversity.
2) Arachis (A.) diogoi introgression lines with immunity to tomato spotted wilt virus will be crossed with a highly susceptible cultivar. Progeny will be genotypes and phenotyped to confirm that specific introgressed regions are associated with resistance.
3) Numerous hybridizations will be made in a systematic manner to develop a 16-parent multi-parent advanced generation intercross (MAGIC) population. Genotyping and phenotyping of progeny will be analyzed to identify Quantitative Trait Loci (QTLs) for multiple disease resistance and quality traits.
4) Results from objectives 2 and 3 will be used to design marker panels for marker-assisted peanut breeding. These panels will be evaluated using MAGIC population or other segregating populations in our breeding program.
