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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Mosquito and Fly Research » Research » Research Project #437033

Research Project: Development of a Combined Sequencing and Data Collection Platform for Simultaneous Vector and Pathogen Detection from Field Surveillance Collections

Location: Mosquito and Fly Research

Project Number: 6036-32000-052-07-I
Project Type: Interagency Reimbursable Agreement

Start Date: May 22, 2019
End Date: Sep 30, 2019

Objective:
The overall objective is to apply improved technology to arthropod surveillance and control. Building on previous work (PN:6036-32000-050-00) we intend to use nanopore sequencing and field portable Real-time polymerase chain reaction to rapidly identify both the arthropod species and potential pathogens based on isolated nucleic acids from mixed field surveillance collection samples. A portion of this project is to test and evaluate the use of a field portable Real-time polymerase chain reaction system which has previously undergone field testing.

Approach:
Collaborators will collect and provide samples of arthropods for analysis. USDA will use the funds to provide equipment and consumables to accomplish the goals of this project, including equipping existing field sequencing kits with field portable Real-time polymerase chain reaction capabilities (BioMeme) to continue platform development. USDA will assist with sequencing analysis, data pipeline development, sequencing data collection and results validation. This will leverage the unique molecular platforms, expertise and skills available within the Mosquito and Fly Research Unit of Center for Medical, Agricultural and Veterinary Entomology.