Project Number: 6040-32000-066-059-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Apr 1, 2019
End Date: Sep 30, 2021
The objectives of this collaborative research are to create a synergy of real-time polymerase chain reaction (PCR) tests for poultry respiratory pathogens and to investigate the multifactorial etiology involving poultry respiratory diseases.
We will conduct a cooperative exercise with select diagnostic laboratories to evaluate by bioinformatics and laboratory testing different diagnostic test for respiratory pathogens, with the goal of identifying the best tests that will be shared with all interested diagnostic laboratories. The selected tests will be compared with existing diagnostic tests on field samples to generate the data needed for diagnostic comparison. It is proposed that diagnostic tests for infectious bronchitis, infectious laryngotracheitis, avian metapneumovirus, and Mycoplasma will be compared through this process. A different pathogen will be evaluated in each year in the project. In the fifth year, proficiency panels will be sent to participating diagnostic laboratories to provide assurance that the tests are being performed on a satisfactory basis. The overall result will the availability of uniform validated diagnostic tests that can be performed by more laboratories that should result in better understanding of disease prevalence and spread in the United States. We will investigate the multifactorial etiology involving poultry respiratory diseases by studying the interactions of relevant respiratory pathogens based on species, their clinical outcomes, transmission patterns, and potential changes in vaccination and diagnostic strategies. Co-infection studies will be conducted in broiler chickens with low pathogenic avian influenza (LPAIV), infectious bronchitis virus (IBV) and Mycoplasma to reproduce bronchial casts and mortality reported with LPAIV infections in the field. Co-infection studies will also be done in turkeys using different respiratory pathogens including LPAIV, NDV, and Mycoplasma. These studies will provide important information on the interaction of respiratory pathogens in chickens and turkeys, which will help improve diagnostics and vaccination strategies needed to control respiratory syndromes.