Location: Crop Germplasm Research
Project Number: 3091-21000-041-08-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 1, 2019
End Date: Jun 30, 2021
The objective of this research is to obtain genotypic and/or genomic data on accessions in the U.S. National Cotton Germplasm Collection and segregating or introgression populations use this data to characterize traits of interest.
The USDA-ARS will provide up to 800 DNA samples to Texas A&M AgriLife for analysis. These samples will be obtained to meet the parameters of the Genomics and Bioinformatics Service Center at Texas A&M AgriLife. The center will provide genotypes and/or sequences for each of the DNA samples. The USDA-ARS will analyze this information and use it in a map-based approach to identify Fusarium oxysporum f. sp. vasinfectum race 4 (FOV4) resistance genes in several experimental cotton populations and parental lines. All parental lines in crosses will be re-sequenced using Illumina technology. Illumina reads will be mapped to the current G. hirsutum reference sequence. SNPs between each parent will be identified. Genetic maps will be created for the segregating F2 populations using Illumina Beadarray or using Genotyping by Sequencing. The project may be extended to other germplasm or traits of interest as agreed upon by both parties.