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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Small Grain and Food Crops Quality Research » Research » Research Project #436541

Research Project: PCHI:Gut Microbiota Dependent and Independent Impacts of Dietary Pulses on Pre/Postprandial Metabolism and Inflammation in Overweight/Obese Humans

Location: Small Grain and Food Crops Quality Research

Project Number: 3060-21650-002-003-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2019
End Date: Aug 31, 2024

Objectives for the life of the project will be to: (1) Determine the impact of pulse (green lentil and black bean) consumption on postprandial triglyceride and inflammation responses to a high-fat meal challenge; (2) Determine the extent to which the gut microbiome and changes in the gut microbiome induced by pulse consumption influence health impacts; and (3) Measure metabolomic profiles to elucidate underlying mechanisms linking pulse consumption to improved health.

We propose two parallel interventions of lentil then black bean versus macronutrient-matched (not including fiber) control treatment for 12 weeks in overwight/obese adults with demonstrated risk for type-2 diabetes and cardiovascular disease. Experimental diets will be provided to participants in the form of pre-made midday meals to exploit the second meal effect and lower caloric intake at the evening meal. Pre- and post-intervention assessments will be made for the following variables: makeup of the gut microbiome (microbial species and relative abundance), gut metabolome, postprandial response of triglyceride, inflammatory cytokines, and serum metabolome to a high-fat meal challenge (established inflammation stimulus), fasting serum glucose, lipid, insulin, inflammation markers and metabolome, blood pressure, and anthropometric measures including weight, body composition, waist circumference, and quantity of visceral adipose tissue.