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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Research Project #436523

Research Project: Spring Black Stem and Stemphylium Leaf Spot Resistance Screening in the USDA-ARS National Plant Germplasm System’s Medicago spp. Genetic Resources

Location: Plant Germplasm Introduction and Testing Research

Project Number: 2090-21000-026-07-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 15, 2019
End Date: Oct 31, 2020

Objective:
1. Optimize inoculation protocols for fungal species causing Spring black stem and Stemphylium lead spot diseases of alfalfa; 2. Systematically screen through alfalfa germplasm for disease resistance to Spring black stem and Stemphylium lead spot; 3. Define host range for Spring black stem and Stemphylium lead spot diseases in subsets of representative Medicago spp. taxa; 4. Evaluate Standard Check variety and inventories for reaction to Spring black stem and Stemphylium lead spot diseases; 5. Make data, and associated information, publicly available through presentations, publications and through the USDA-ARS National Plant Germplasm System’s GRIN-Global database.

Approach:
Alfalfa and relatives (Medicago spp.) germplasm will all be requested from PGITRU seed storage facility and grown in disease-free greenhouses. Fungal plant pathogen inoculum will be grown using standard microbiological procedures in laboratories. Inoculations will be performed in head-houses and inoculated plants grown in containment greenhouses. Plants will be scored for disease reaction and all material to be discarded will be sterilized. Resistant germplasm will be propagated for further evaluations.