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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #436421

Research Project: Discovering New Metabolic Constraints and Regulatory Nodes in Oilseeds Engineered for Enhanced Fatty Acid Synthesis and Seed Oil

Location: Plant Genetics Research

Project Number: 5070-21000-045-001-N
Project Type: Non-Funded Cooperative Agreement

Start Date: Nov 1, 2018
End Date: Oct 31, 2023

The overall objective of the current application is to use a systems level approach including 'omics' and flux analysis to pinpoint altered regulation in high oil producing lines of multiple oilseed species.

'Multi-omic' data including: transcripts, metabolite profiling, proteomics, radioactivity assays, 13C-metabolic flux analysis and both kinetic and empirical modeling will be used to probe the changes in central and lipid metabolism that are responsible for production of significantly more oil in lines of soybean, camelina and Arabidopsis. The multiple 'omics' evaluations will be leveraged to find bottlenecks in metabolism. Kinetic models will mechanistically show at what 'omic' level the control of fatty acid biosynthesis for lipid production occurs in these seeds.