Location: Forage and Range Research
Project Number: 2080-21000-018-032-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Oct 1, 2018
End Date: Aug 31, 2022
Complete six consecutive generations of inbreeding at full-sib level using marker assisted selection.
Previously developed alfalfa genotypes from salt and drought tolerant populations will be used as source material. From among them will be identified 200 genotypes for inbreeding. It is anticipated that from each of these 200 genotypes 25 inbred lines will be successfully developed following six generations of full-sib mating. During the inbreeding process, progeny from these populations will be phenotyped for seed production and forage nutritive value partiularly lignin content. High and low extreme individuals for each trait will be identified and pooled for bulked segregant analysis. Multiple Bulked Segregant Analysis (BSA) will be used to map genes controlling inbreeding compatibility and lignin content. This will in turn be used for marker assisted selection (MAS), toward achieving the goal of six consecutive generation of inbreeding at full-sib level. Total plant samples will be collected, dried and ground using standard procedures for forage near-infrared reflectance spectroscopy (NIR) analysis. Forage nutritive value will be determined using near-infrared reflectance spectroscopy specifically acid detergent lignin with inhouse equations. System software will be used to select samples for wet chemistry analysis and validations. Separate plant tissue samples will be collected from each genotype for molecular analysis conducted at Washington State University.