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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Research Project #436124

Research Project: Identification of Proteins Involved in Cross Protective Immune Responses as a Mechanisms for Broadly Protective Bacterial Vaccine Development

Location: Virus and Prion Research

Project Number: 5030-32000-119-015-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Apr 1, 2019
End Date: Apr 1, 2020

Use immunoproteomics for the identification of proteins involved in broadly protective immune responses of common bacterial pathogens of swine.

Aim 1: Outer membrane proteins will be isolated from H. parasuis and two dimensional gel electrophoresis will be run to separate the outer membrane proteins and proteins of interest will be selected from the identified proteins. Aim 2: Whole cell lysate from H. parasuis will be incubated with antibody generated by protected animals and non-protected animals. The proteins bound by the sera will then be eluted from the antibody and separated using two dimensional gel electrophoresis and characterized by mass spectrometry. Aim 3: Proteins identified in Aim 1 and Aim 2 will be produced as recombinant proteins. A vaccine trial will then be completed to test the efficacy of the identified proteins as a subunit vaccine candidate.