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ARS Home » Southeast Area » Raleigh, North Carolina » Soybean and Nitrogen Fixation Research » Research » Research Project #436121

Research Project: Protein-based Thermotolerance Markers for Sustainable Legume Protein Production

Location: Soybean and Nitrogen Fixation Research

Project Number: 6070-21220-069-031-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Feb 1, 2019
End Date: Jan 31, 2021

Objective:
1. Identify post-translational regulatory networks that govern responses to early-season cold and late-season heat stress; 2. Predict phenotype trajectories from molecular data using machine learning; and 3. Identify and validate temperature associated, protein-based breeding markers for soybean and other legumes.

Approach:
In this project, we will identify post-translational protein modifications that improve yield and seed protein content in soybean following periods of high or low temperature stress. We will accomplish this using advanced network analysis algorithms and machine learning to link molecular responses with agronomic results. The ARS investigator will conduct growth chamber and field experiments, physiological analyses, assist with data analysis, and manage the project as PI. Tissue will be sent to VIB for phosphoproteomic analysis, and data will be modeled by North Carolina State University. The funds from BASF will be put toward heated field plots for field validation of phosphomarkers for temperature stress.