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ARS Home » Southeast Area » Mississippi State, Mississippi » Crop Science Research Laboratory » Genetics and Sustainable Agriculture Research » Research » Research Project #435840

Research Project: Evaluation of Tri-species Chromosome Substitution Lines (CS) with Novel Alleles for Improved Fiber Quality Traits (CI project 16-349)

Location: Genetics and Sustainable Agriculture Research

Project Number: 6064-21000-016-12-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Jan 1, 2018
End Date: Dec 31, 2018

Objective:
Create and evaluate an unique set of germplasm by targeted introgression of desirable genes from three tetraploid species including wild and unadapted types using chromosome substitution lines of Gossypium (G.) barbadense, G. tomentosum and G. mutslinum for improved productivity of Upland cotton in diverse environments.

Approach:
The recurrent parent TM-1 and the selected Chromosome Substitution (CS) lines specific to chromosome 17 and chromosome 18 of Gossypium (G.) mustelinum (CS-M), Gossypium barbadense (CS-B) and G. tomentosum (CS-T) will be used for field evaluation of agronomic and fiber traits at three locations (USDA/ARS, Mississippi State, USDA/ARS, Florence, South Carolina, and USDA/ARS, Lubbock, Texas) in replicated trial plots in summer of 2015. A 25-boll hand-harvested seedcotton sample will be collected from each plot prior to machine picking. These samples will be weighed to determine boll size and ginned on a laboratory 10-saw gin to determine lint percentages and to provide lint samples for fiber analyses. Fiber samples will be sent to Cotton Incorporated for HVI quality determination. Yield will also be determined. Direct comparisons among the lines and TM-1 will be used to detect effects of the substituted chromosommes on important characteristics. We will use an additive-dominance (AD) with G*E interaction genetic model for data analysis to determine the effects of the substituted chromosome or chromosome arms on fiber and agronomic traits following our previous studies. Three quarterly and one annual report will be sent in electronic WORD format to the project manager.