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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Research Project #435829

Research Project: Host Plant Resistance and Characterization of Genes Involved in Disease Resistance

Location: Plant Stress and Germplasm Development Research

Project Number: 3096-21000-022-18-A
Project Type: Cooperative Agreement

Start Date: Nov 1, 2018
End Date: Jan 30, 2019

Objective:
To assist in seed production and evaluations of selected entries and characterize candidate genes involved in plant stress and disease resistance/tolerance.

Approach:
Cotton (Gossypium spp.) breeding lines and additional progeny of interest developed and identified from ongoing screening and selection efforts from a cooperative project by the USDA-ARS, CSRL, Plant Stress and Germplasm Development Research (PSGDR), TX and University cooperators are being evaluated in infested fields using RCBD or incomplete block designs with 3–4 replications. These improved lines target introgression of resistance/tolerance genes from diverse genetic backgrounds. Multiple evidences from model and crop species demonstrated that the cell wall acts as a physical barrier against pathogen infection. Breeding lines will be used to isolate cell walls and analyze its composition using a histochemical analysis of the cotton root cross sections using staining various cell wall polymers. Lines with higher cell wall polymers will be further analyzed for the biochemical composition by wet lab methods (HPLC/GC/Spectrophotometer). In addition, research carried out at the USDA-ARS, PSGDRU and Texas Tech University Center for Biotechnology and Genomics (CBG) has generated genomic information of expressed genes from FOV4 infection plant using next generation sequencing (NGS). From this USDA-ARS ongoing RNA-Seq transcriptomic research, candidate genes involved in cell wall fortification will be identified. In addition, the assembled two ancestral diploid parents (G. raimondii, D5 and G. arboreum, A2), and tetraploid Upland (G. hirsutum) and Pima (G. barbadense) cotton genome reference-sequences will also be used to align of selected gene sequences to the genome references. This will provide chromosomal map position and SNPs for genotyping and marker assistance selection or MAS. The developed SNP biomakers can be used for genetic/genomic studies and assisting breeding germplasm with improved SNP-biomarkers will eventually be used in marker-assisted breeding for developing tolerant cotton lines.