Location: Meat Safety and Quality
Project Number: 3040-42000-017-003-T
Project Type: Trust Fund Cooperative Agreement
Start Date: Oct 1, 2018
End Date: Sep 30, 2019
Use whole genome sequencing and in vitro laboratory HGT experiments on E. coli and Salmonella isolates obtained from commercial feedlots to compare in vitro laboratory HGT levels to in situ results.
Whole genome sequencing (WGS) will be performed on at least 400 isolates (distributed proportional to their isolation frequency) of cephalosporin-resistant (CR) Salmonella, azithromycin-resistant (AR) Salmonella, susceptible Salmonella, AR E. coli, and CR E. coli, isolated during longitudinal studies of cattle feeding operations. Dominant strains or sub-types of CR Salmonella, AR Salmonella, susceptible Salmonella, AR E. coli, and CR E. coli at each cattle production site will be identified and used to perform in vitro laboratory horizontal gene transfer (HGT) experiments.