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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Research Project #435740

Research Project: Targeting a Conserved Structural Model in Phytophthora Effectors for Disease Resistance

Location: Vegetable Crops Research

Project Number: 5090-21220-005-21-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Sep 1, 2018
End Date: Aug 31, 2021

Objective:
Evaluate a hypothesis that the conserved structure of L-W-Y domain provides a basic interface that allows effector interaction with common host components, which may be required for effector functions in plant cells.

Approach:
We will use greenhouse and laboratory screening approaches to identify wild potato germplasm that can recognize and elicit a resistance response after exposure to specific pathogen effector proteins. We will then use genetic and genomic approaches to identify candidate genes involved in the recognition response. 1. Characterize a common protein that interacts with multiple L-W-Y motif-containing effectors for its role in effector function. Its role in effector function will be investigated. 2. Identify potential resistance genes that recognize the L-W-Y domain. We will screen wild Solanaceae species for those that can activate hypersensitive response to multiple LWY- containing effectors. These wild Solanaceae species will then be analyzed by enriched high-throughput sequencing techniques to identify responsible Resistance genes.