Location: Vegetable Crops Research
Project Number: 5090-21220-005-21-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Sep 1, 2018
End Date: Aug 31, 2021
Evaluate a hypothesis that the conserved structure of L-W-Y domain provides a basic interface that allows effector interaction with common host components, which may be required for effector functions in plant cells.
We will use greenhouse and laboratory screening approaches to identify wild potato germplasm that can recognize and elicit a resistance response after exposure to specific pathogen effector proteins. We will then use genetic and genomic approaches to identify candidate genes involved in the recognition response. 1. Characterize a common protein that interacts with multiple L-W-Y motif-containing effectors for its role in effector function. Its role in effector function will be investigated. 2. Identify potential resistance genes that recognize the L-W-Y domain. We will screen wild Solanaceae species for those that can activate hypersensitive response to multiple LWY- containing effectors. These wild Solanaceae species will then be analyzed by enriched high-throughput sequencing techniques to identify responsible Resistance genes.