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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Research Project #435694

Research Project: Spring Black Stem and Stemphylium Leaf Spot Resistance Screening in the USDA-ARS National Plant Germplasm System’s Medicago spp. Genetic Resources

Location: Plant Germplasm Introduction and Testing Research

Project Number: 2090-21000-026-02-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jan 1, 2019
End Date: Jun 30, 2020

Objective:
1. Optimize inoculation protocols for fungal species causing Spring black stem and Stemphylium lead spot diseases of alfalfa. 2. Systematically screen through alfalfa germplasm for disease resistance to Spring black stem and Stemphylium lead spot. 3. Define host range for Spring black stem and Stemphylium lead spot diseases in subsets of representative Medicago spp. taxa. 4. Evaluate Standard Check variety and inventories for reaction to Spring black stem and Stemphylium lead spot diseases. 5. Make data and associated information publicly available through presentations, publications and through the USDA-ARS National Plant Germplasm System’s Germplasm Resource Information Network (GRIN) Global database.

Approach:
Alfalfa and relatives (Medicago spp.) germplasm will all be requested from Plant Germplasm Introduction and Testing Research Unit (PGITRU) seed storage facility and grown in disease-free greenhouses. Fungal plant pathogen inoculum will be grown using standard microbiological procedures in laboratories. Inoculations will be performed in head-houses and inoculated plants grown in containment greenhouses. Plants will be scored for disease reaction and all material to be discarded will be sterilized. Resistant germplasm will be propagated for further evaluations.