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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Research Project #435644

Research Project: The Toxisome: An Organelle for Terpene Synthesis Involved in Fungal Virulence

Location: Cereal Disease Lab

Project Number: 5062-21220-022-08-I
Project Type: Interagency Reimbursable Agreement

Start Date: Sep 1, 2018
End Date: Aug 31, 2020

Objective:
1) Characterize the protein content and arrangement of the toxin biosynthetic apparatus in the Fusarium head blight pathogen Fusarium graminearum; 2) Test for differentiation of the endoplasmic reticulum for specific primary and secondary terpenoid metabolite pathways upon toxin induction.

Approach:
Our approach will be to identify components of the toxin biosynthetic apparatus by tagging proteins with fluorescent proteins and then localization using confocal- or superresolution microscopy. Protein-protein interactions will be accessed using fluorescence resonance energy transfer utilizing acceptor photobleaching. Site directed mutagenesis will allow for measuring the effect of individual proteins on toxin accumulation.