Project Number: 5020-21220-019-10-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2018
End Date: Aug 31, 2020
To identify molecular markers linked to new and previously known genes for resistance to Septoria tritici blotch and other diseases in wheat.
In this project previously characterized populations of elite wheat breeding lines and historical wheat cultivars will be phenotyped in the field for resistance to Septoria tritici blotch and any other diseases that are present. Natural inoculum will be used for most studies and plants will be scored after the flag leaves on most cultivars are fully developed and disease symptoms are occurring. The same populations also will be grown in a greenhouse and inoculated with one or more individual isolates of the pathogen to look at seedling resistance and to test for specific interactions with particular isolates. Artificially inoculated plants will be scored for disease approximately three weeks after inoculation. In all cases plants will be scored for pycnidial density and percent of leaf area infected by visual estimation. Leaves also may be collected and scored for the same parameters using an automated image-analysis system. Phenotypic data will be combined with previously obtained genotypic data from the same lines and associations identified with standard GWAS statistical analyses, to identify genes closely linked to resistance loci.