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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Bioenergy Research » Research » Research Project #435501

Research Project: Harnessing the Robustness of Undomesticated Yarrowia lipolytica Strains for Biosynthesis of Designer Bioesters

Location: Bioenergy Research

Project Number: 5010-41000-189-002-I
Project Type: Interagency Reimbursable Agreement

Start Date: Sep 1, 2018
End Date: Aug 31, 2024

Develop oleaginous yeasts that produce medium chain carbon esters for use in biodiesel and solvent applications.

We will generate a mixture of sugars from lignocellulose by pretreating the biomass with dilute-acid at 160-180 degrees Celsius for 10-30 minutes, neutralizing the hydrolysate, and extracting the fermentable sugars using commercial cellulases. The sugar streams will be used for biological conversion to biodiesel using engineered oleaginous yeasts in aerobic cultures. Production will be optimized by adjusting the dilution of the hydrolyate, adjusting culture conditions (pH, temperature, and mixing), medium composition (nitrogen source, macro-element concentrations, and the carbon to nitrogen ratio), and finally utilizing complex culture strategies possibly including staged and fed-batch cultures. Where suitable statistical experimental designs, factorials and response surface(s), will be implemented for eliciting important factors and determining optimal culture conditions.