Location: Diet, Genomics and Immunology Laboratory
Project Number: 8040-51530-057-11-I
Project Type: Interagency Reimbursable Agreement
Start Date: Aug 13, 2018
End Date: Aug 12, 2019
Citobacter rodentium is a murine pathogen causing transmissible colonic hyperplasis and colities with a pathogenic mechanism similar to foodborne enterohaemorrhagic Escherichia coli in humans. ARS are going to use mice infected with these bacteria as a model to examine potential role of I3C on inflammatory pathways in vivo.
Experiment 1 Examine how I3C enhances intestinal regulatory T (Treg) cell function in C rodentium infected mouse model. Tregs are essential negative regulators of inflammation. ARS hypothesized that I3C could protect against C rodentium-induced inflammation. Therefore, we will evaluate the effect of I3C on the relevant peripheral immune-regulating mechanisms in C rodentium-infected mice. Experiment 2 Evaluate how I3C/DIM attenuates LPS-induction of PDL 1 in human THP-1 macrophages. In previous studies, ARS showed that PDL-1 expression was inducted with LPS stimulation in THP-1 human macrophage cells. And our preliminary data in vitro suggest that I3C/DIM may interfere with LPS-induced PDL-1 expression. On the basis of these data, we plan to determine the role of I3C/DIM in blocking immune check-points in the context with cancer prevention.