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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Research Project #435090

Research Project: Managing Fungicide Resistance in Botrytis Cinerea on Wine and Table Grape, Strawberry, and Blueberry

Location: Crop Diseases, Pests and Genetics Research

Project Number: 2034-21220-007-16-A
Project Type: Cooperative Agreement

Start Date: Sep 1, 2018
End Date: Jan 31, 2020

Objective:
The objective of this work is to collect and characterize fungicide sensitivity of Botrytis cinerea collected from specialty crops in California. In addition, genetic diversity of the fungus will be evaluated, and diagnostic markers for detecting fungicide resistant isolates will be developed.

Approach:
Fruit will be collected from grape, strawberry, blueberry fields and packing houses across the San Joaquin Valley and Central Coast of California. Botrytis cinerea will be isolated from fruit and single spored for phenotypic (virulence and fungicide resistance to 7 compounds) and molecular analyses. AmpSeq will be used to identify population diversity and genomic regions associated with fungicide sensitivity. Diagnostic markers will be developed to detect fungicide-resistant isolates using AmpSeq results.