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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Food Components and Health Laboratory » Research » Research Project #434984

Research Project: Phase II - Analytical Phase of The Dose-Response Effects of Lean Beef in a Mediterranean-Style Dietary Pattern on CVD Risk Factors

Location: Food Components and Health Laboratory

Project Number: 8040-51530-011-10-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Aug 1, 2018
End Date: Sep 30, 2019

To conduct Phase II of a study to evaluate established risk factors and emerging biomarkers of cardiovascular disease of a Mediterranean-style dietary pattern that will provide three quantities of lean beef in a dose-dependent manner (0.5 oz. 2.5 oz. and 5.5 oz. per day). In our diet design, we will provide the specified amount of lean beef for each test diet plus the remaining amount of protein equivalents from other protein foods (i.e., fish, poultry, pork, nuts, eggs, legumes) to achieve the target (7 oz. per day) and a protein content of ~17% of energy for the Mediterranean-style dietary patterns with beef.

Using a multicenter approach with two participating centers (Penn State University and USDA-Beltsville), we will conduct a 4-period randomized crossover controlled-feeding study. Subjects (n = 60; 30 at each Center) will be randomly assigned to receive each 4-week test diet in random order. The four test diets are: 1) a Mediterranean diet (MED 0.5) providing 0.5 oz. per day of lean beef; 2) a matched MED diet (MED 2.5) that will have the same fatty acid profile as the MED diet, but will contain 2.5 oz. per day of lean beef; 3) a matched MED diet (MED 5.5), providing 5.5 oz. per day of lean beef (on a 2100 calorie diet); and 4)an Average American Diet (AAD). Endpoints will include biomarkers of cardiovascular disease. Phase II will focus on analytical and post-analytical work associated with the dietary intervention.