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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » Research » Research Project #434972

Research Project: Interaction of a Long-acting Eprinomectin Product with BM86 Vaccination Against Cattle Fever Ticks

Location: Livestock Arthropod Pests Research

Project Number: 3094-32000-039-94-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 1, 2018
End Date: Jun 30, 2020

Objective:
Complete concentration analysis of eprinomectin in animal serum collected in support of BM86/eprinomectin interaction studies using a long-acting eprinomectin product against cattle fever ticks.

Approach:
Determine if an interaction exists with the combination of a long-acting eprinomectin injectable with anti-tick vaccination in support of its potential use in the USDA APHIS VS Cattle Fever Tick Eradication Program. This project will consist of two field studies. Both will be in pastures located at the USDA ARS Cattle Fever Tick Research Laboratory in Edinburg, TX. For the first field trial, cattle will be infested with known amounts of tick larvae at regular intervals. A quarter of the animals will be treated at the label rate with long-acting eprinomectin injectable, another quarter will receive an anti-tick vaccination, a third quarter will receive both eprinomectin injectable and anti-tick vaccination. The remaining quarter will serve as untreated controls. Regular tick infestations will occur. Tick counts will be made weekly. Up to 10 engorged females will be collected from each animal and held in an incubator for fecundity and fertility determinations. The study will terminate when efficacy reaches below 90% for three consecutive days. The second field study cattle will be artificially infested with larvae twice per week for 10 weeks to establish a sustainable active field infestation of ticks. All animals will then be treated with a long-acting eprinomectin twice at monthly intervals and vaccinated with BM86 at day 0 and 28. Tick counts will be made weekly. The study will terminate when tick numbers rise to an average of 30 standard females per animal or 180 days post initial treatment. Throughout both studies blood will be collected from all animals at regular intervals. Serum will be sent to the collaborator for determination of eprinomectin concentration analysis. ELISA and immunology data will be analyzed by the collaborator.