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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Research Project #434945

Research Project: Acquisition and Establishment of Rose Virus Positive Control Collection

Location: National Germplasm Resources Laboratory

Project Number: 8042-22000-302-15-I
Project Type: Interagency Reimbursable Agreement

Start Date: Oct 1, 2017
End Date: Sep 30, 2019

Objective:
A. Acquire rose varieties infected with known viruses to establish a curated rose virus collection. B. Utilize and compare established diagnostic protocols to test infected roses before material is added to the virus collection. C. Make material from the collection available to the National Clean Plant Network and National Plant Diagnostic Network to use as positive controls in diagnostic tests.

Approach:
The collaborators will obtain rose samples with virus-like symptoms from several nurseries and gardens that have already agreed to donate material. Each sample will be independently tested by ARS, Texas A&M, and FPS/Univeristy of California-Davis using electron microscopy, PCR, and high throughput sequencing,respectively. The goal is to obtain and curate high virus-titer specimens of each of the approximately 15 known rose viruses, with as as many single infection specimens as possible. Criteria will be established to select and curate secimens as a standardized rose virus positive control collection, and make samples from it freely available to stakeholders, including National Clean Plant Network Centers and National Plant Diagnostic Network laboratories. All PIs will be involved in extension, outreach, and education as part of industry presentations, distributing diagnostic protocols, and publication of results.