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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Genetic Improvement for Fruits & Vegetables Laboratory » Research » Research Project #434905

Research Project: Development of Diploid Potato Germplasm for Nitrogen Use Efficiency to Reduce Fertilizer Use and Ground Water Contamination

Location: Genetic Improvement for Fruits & Vegetables Laboratory

Project Number: 8042-21000-283-02-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 1, 2018
End Date: Sep 30, 2019

Objective:
1) Determine the amount of variation for potato tuber yield and specific gravity under high and low nitrogen rates in progeny resulting from the cross of BD1211-1 and Bchc0048-24. 2) Identify offspring with superior nitrogen uptake efficiency (NUE) that produce 2n pollen, and make 4x-2x crosses to move NUE into the tetraploid germplasm base.

Approach:
1) Progeny clones will be evaluated for tuber yield under high and low N fertilization in the field in Florida and Maine in 2019. The experimental design will be a split-plot with 2-3 replications, depending on seed increase in 2018. The main-plot is fertilizer applied (high, low) and the sub-plot is clone. Otherwise, common commercial practices will be employed at both sites. Each sub-plot will consist of 5-10 plants of a clone. At harvest, tubers from the plots will be counted and weighed. A tuberization efficiency index (TEI) for each clone will be calculated as the average tuber yield under low N divided by the average tuber yield under high N. Specific gravity will be determined for each plot using the weight in air, weight in water method. 2) Pollen of promising NUE clones will be stained and examined under the microscope for 2n pollen. Those clones that produce 2n pollen will be crossed with tetraploid germplasm to incorporate NUE into the tetraploid population.