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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Genetic Improvement for Fruits & Vegetables Laboratory » Research » Research Project #434903

Research Project: Identifying Genes for Late Blight, Early Blight and Common Scab Resistance and Processing Traits in a Tetraploid Potato Mapping Population

Location: Genetic Improvement for Fruits & Vegetables Laboratory

Project Number: 8042-21000-283-01-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 1, 2018
End Date: Oct 30, 2019

Objective:
Evaluate the B3353 population for resistance to late blight, early blight and common scab. Evaluate the B3353 population for maturity, yield, specific gravity, and chip color. Identify genes governing late blight, early blight and common scab resistance and processing traits. Develop markers for marker-assisted selection efforts to facilitate potato molecular breeding.

Approach:
The 230 clones from the B3353 population and their parents will be evaluated for late blight and early blight resistance in separate disease nurseries in Rock Springs, PA. The experimental design will be a randomized complete block design with two replications and five-hill plots per clone. Disease inoculum will be applied if conditions do not favor natural disease development. Clones will be evaluated several times during the growing season for late blight and early blight, area under the disease progress curve will be calculated, and analyzed using the general linear models procedure in SAS. This population will be evaluated for common scab resistance in later years in Rock Springs, PA when enough seed are available. These same clones and their parents will be planted in Presque Isle, Maine in a randomized complete block design with two replications and 20-hill plots per clones. Clones will be evaluated for maturity, based on flowering mid-season. Following harvest, data on yield and tuber size distribution will be collected during the grading process, specific gravity will be recorded, and five-tuber chip samples will be placed in 100C storage for processing three months later. Tuber samples will be fried in January and chip color recorded. Yield, specific gravity, and chip color data will be analyzed using the general linear models procedure in SAS. Total genomic DNA has already been extracted and purified using the DNeasy Plant Mini Kit. Genotyping will be performed using the Illumina Solanaceae Coordinated Agricultural Project (SolCap) potato single-nucleotide polymorphism (SNP) array. Genome loci significantly associated with late blight, early blight and common scab resistance, maturity, yield, specific gravity and chip color will be identified. SNPs linked to these traits will be identified and will be used as markers for future selection.