Location: Poultry Research
Project Number: 6064-13000-013-000-D
Project Type: In-House Appropriated
Start Date: Jul 7, 2018
End Date: Sep 30, 2022
1. Use proteomics, genomics, and systems biology approaches to identify molecular determinants of pathogenesis, strain variation, and tissue tropism of different Escherichia (E.) coli strains. 2. Identify immunological targets that will confer cross-protection against prevalent E. coli strains in poultry production and develop vaccine platforms that are effective in very young birds, provide cross-protection, and can be easily administered. 3. Develop systems-level capabilities to evaluate the effects of commercial-scale, poultry management practices on animal health and production; microbial ecology, development of antimicrobial resistance and bacterial pathogen transmission to develop mitigation strategies.
Escherichia (E.) coli is a bacterium that commonly inhabits the gastro-intestinal tract of most animals including poultry. While most strains are nonpathogenic, virulent E. coli strains are often associated with disease in poultry (e.g. colibacillosis) and result in significant economic losses to the poultry industry. To combat E. coli-associated disease in poultry, genotypic and phenotypic traits of virulent E. coli isolates associated with previous colibacillosis outbreaks in chickens will be characterized. The knowledge derived from isolate comparisons will be applied to characterization of E. coli-associated disease as well as to the development of a novel and effective vaccine. Further, vaccination platforms will be developed to maximize the delivery of commercially-available and research derived-vaccines. Application techniques to be investigated will include the traditional means of vaccine delivery including spray, drinking water, and eye-drop. However, novel delivery techniques such as in ovo injections will also be assessed for efficient vaccine delivery. In addition, investigations will be performed to determine the impact of poultry management practices on mitigation of E. coli-related disease. Areas of mitigation interest will include practices to limit E. coli transmission rates and the survivability/maintenance of the pathogen within the poultry environment. The impact of dietary additives including prebiotics, probiotics and bacteriophage will also be considered as means to limit the occurrence of E. coli-related disease in poultry.