Location: Corn Insects and Crop Genetics Research
Project Number: 5030-22000-019-02-N
Project Type: Non-Funded Cooperative Agreement
Start Date: Oct 1, 2017
End Date: Sep 30, 2020
This study aims to define the level and geographic range of pyrethroid resistance in Iowa. Furthermore, the biochemical and genetic basis of pyrethroid resistance will be investigated.
Field populations will be collected from several locations across NW Iowa, and then screened in the laboratory for running diagnostic bioassays that will estimate the frequency and fold-resistance compared to a susceptible baseline colony. The genomic basis of pyrethroid resistance will be investigated by the application of next generation sequencing (NGS) technologies by re-sequence pools of resistant and susceptible individuals from two different locations. Mutations that are fixed or showing a high frequency differences between resistant and susceptible pools will be identified computationally, and genes or genome regions containing these mutations will be identified for further downstream research.