Project Number: 8010-22000-028-28-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 15, 2018
End Date: Nov 30, 2019
The goal of the proposed research is to evaluate the host specificity of the native North American parasitoid (O. millipes) and then determine the potential efficacy and laboratory adaption of these parasitoids reared on ALB larvae in suppressing ALB populations under field-cage and/or open-field conditions. Specifically, we will (a) conduct quarantine laboratory testing of the native nontarget longhorned beetles with O. millipes and (b) conduct laboratory, field-cage and/or field cage testing testing with O. millipes to evaluate its potential for laboratory adaption and its efficacy in suppressing ALB populations.
To assess the host specificity of O. mellipes, we will focus on testing common species of native North American longhorned beetles (Coleoptera: Cerambycidae) infesting major hardwood trees such as maple, birch, and oak. Live adults of different species of North American cerambycids will be collected between May and August each year from the hardwood forests in mid-Atlantic and/or Northeast United States using longhorned beetle traps developed by USDA APHIS Otis laboratory. Adults of different species of native longhorned beetles collected from the traps will then be reared with foliage or twigs of their preferred host plants and sugar water and provided with host logs or other substrates for oviposition at the USDA APHIS Otis laboratory or the USDA-ARS facility in Newark, DE. Native longhorned beetle larvae will be reared on artificial diet until they are of a size comparable to the size of ALB parasitized by O. mellipes (instar 2-3). Once a suitable size is reached, the larvae will be inserted in logs of the appropriate host tree for testing. We will expose gravid females of O. mellipes to suitable larval stages (2 – 3rd instars) of different species of native North American cerambycids using the similar procedures described by Duan et al (2015) and Golec et al. (2016). We are targeting testing the major native longhorned beetle species infesting maple, oak, and/or birch trees and determine the potential native host range of O. mellipes. Following host specificity testing, we will conduct series of experiments under field-cage or open field conditions in ALB-infested (quarantined) areas (Wooster, MA and Clement, OH) to evaluate the efficacy of laboratory-reared, ALB-adapted O. mellipes in parasitizing ALB larvae infesting maple logs or live trees. Because host specificity testing will take one to two years depending on success in collecting and rearing beetles, field tests will not occur in 2018. USDA APHIS PPQ Otis laboratory has developed trapping and rearing methods for many native species of longhorned beetles and will be providing eggs or larvae of the nontargted longhorned beetles for host-specificity testing with O. mellipes at USDA ARS BIIRU. For detailed approaches, please see attached work plan.