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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Research Project #434745

Research Project: FB6.0331 FF Non-transgenic Genetic Sexing Systems

Location: Tropical Crop and Commodity Protection Research

Project Number: 2040-22430-026-36-I
Project Type: Interagency Reimbursable Agreement

Start Date: Oct 1, 2017
End Date: Oct 30, 2021

Objective:
1. Screen the genomes of existing medfly genetic sexing strains to characterize the mutations causing the temperature sensitive lethal and white pupae traits. 2. Characterize the exact translocation breakpoint between the Y chromosome and the 5th autosome in the males of the Veinna-8 strain that confers these traits to be female specific. 3. Adapt CRISPR-based methods to recreate the translocation in wild type lines. 4. Demonstrate a targeted gene editing approach to re-creating temperature. sensitive lethal in Anastrepha and Bactrocera species.

Approach:
The overall concept of this proposal is to first characterize the mechanism of the existing genetic sexing system in the medfly Vienna-8 strain using forward genetic techniques, validate those methods through reverse genetic screens using targeted gene editing techniques, and then re-create orthologous mutations in wild type lines of Bactrocera and Anastrepha for which robust genetic sexing strains do not currently exist. This is outlined in the following objectives.