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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Research Project #434733

Research Project: Development and Implementation of High Throughput Phenotyping Platforms for the Quantification of Root System Architecture

Location: Plant, Soil and Nutrition Research

Project Number: 8062-21000-046-13-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jun 1, 2018
End Date: Jul 31, 2020

Objective:
The primary objectives of this research are: 1) To continue to improve the throughput and image quality of the current 2-D pouch system; 2) Develop new platforms suitable for the growth, and image acquisition of Arabidopsis roots, for 2D and 3D root architecture analysis.

Approach:
1) For objective 1, improvements in the optical root imaging system will be in the post-imaging software, to improve thresholding and skeletonization of images, in order to be able to better identify fine roots. An assessment of the suitability of these imagining platforms across different plant species and growth media will be performed. 2) For objective 2, based on the existing platforms’ designs, a new platform for imaging smaller and dinner root systems form Arabidopsis roots will be developed. The development will require assessing new imaging and growing conditions under non-sterile conditions (commonly used to grow Arabidopsis) suitable for the implementation of 2D – mini- pouch systems, as well as for 3D imaging. The suitability of transparent soils (namely Nafion, an heterogeneous transparent polymer substrates) will be evaluated by comparing the Arabidopsis RSA grown in this substrate, to that of roots grown in soil, and those commonly grown in sterile agar media supplemented with nutrients and sucrose.