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ARS Home » Pacific West Area » Davis, California » Crops Pathology and Genetics Research » Research » Research Project #434641

Research Project: Application of Forward and Reverse Genetics to Rice Improvement (continuing project)

Location: Crops Pathology and Genetics Research

Project Number: 2032-21000-023-04-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jan 1, 2018
End Date: Mar 31, 2019

Objective:
Research will be conducted on the identification and characterization of rice plants with novel productivity and grain quality traits by screening populations of rice mutants, determining the molecular genetic basis for any novel traits identified in those populations, and developing DNA markers for the transfer of those traits to elite rice cultivars. Specific objectives include: 1) Complete evaluation of arsenic and silicon content of mutant lines grown in the field in 2017; 2) Develop lsi1/lsi2 double mutants; 3) Evaluate new lsi1 and Osabcc1 mutants; 4) Evaluate herbicide tolerance and development of rice mutants identified by TILLING.

Approach:
Rice mutants identified by TILLING and confirmed by Sanger sequencing will be evaluated for various target traits. Silicon and arsenic content of rice shoot tissue and brown rice of mutants grown under field conditions will be determined using UC Davis Analytical Lab services. Double mutants will be generated by genetic crossing and verified by molecular markers. Additional mutants identified by TILLING in 2017 will be confirmed and evaluated for silicon, arsenic and germanium uptake. Other mutants will be evaluated for their response to various herbicides. These evaluations will include lab, greenhouse and field-based assays.