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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Commodity Protection and Quality Research » Research » Research Project #434551

Research Project: Improving and Verifying Quality of Mass-Reared Navel Orangeworm for Sterile Insect Technique

Location: Commodity Protection and Quality Research

Project Number: 2034-43000-040-53-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Apr 1, 2018
End Date: Mar 31, 2019

Objective:
The objectives of this proposal are to 1) examine effects of transportation conditions (temperature, darkness) on Navel Orangeworm (NOW) shipped from the Phoenix rearing facility to California, 2) use a field assay to document sterile NOW competitiveness with local wild males, and 3) document dispersal of sterilized NOW males and females in large-scale field releases.

Approach:
To meet the objectives we will (1) Use flight cylinder (a laboratory behavioral assay) to compare the performance of California or Phoenix strain moths in either the presence or absence of exposure to shipping (cold, darkness) and irradiation. (2) Use an assay with unmated females in a small isolated field plot to determine the impact of released sterile males on reduction of mating with wild males. (3) Use a grid of pheromone traps in a large field site to compare distance flown and percent recaptured between irradiated Phoenix moths and non-irradiated Parlier moths.