Research Project: Immunodiagnostics to Detect Prions and Other Important Animal Pathogens

Location: Produce Safety and Microbiology Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Sensitive and selective detection of peanut allergen Ara h 1 by ELISA and lateral flow immunoassay - (Peer Reviewed Journal)
Hnasko, R.M., Lin, A.V., McGarvey, J.A., Mattison, C.P. 2022. Sensitive and selective detection of peanut allergen Ara h 1 by ELISA and lateral flow immunoassay. Food Chemistry. 396. Article 133657. https://doi.org/10.1016/j.foodchem.2022.133657.

Chronic wasting disease (CWD) in cervids and the consequences of a mutable protein conformation - (Peer Reviewed Journal)
Silva, C.J. 2022. Chronic wasting disease (CWD) in cervids and the consequences of a mutable protein conformation. ACS Omega. 7(15):12474–12492. https://doi.org/10.1021/acsomega.2c00155.

Detecting differences in prion protein conformation by quantifying methionine oxidation - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L. 2022. Detecting differences in prion protein conformation by quantifying methionine oxidation. ACS Omega. 7(3):2649-2660. https://doi.org/10.1021/acsomega.1c04989.

The challenges of detecting an evolving prion disease, chronic wasting disease (CWD) - (Abstract Only)
Silva, C.J. 2022. The challenges of detecting an evolving prion disease, chronic wasting disease (CWD)[Abstract]. American Chemical Society National Meeting. https://doi.org/10.1021/scimeetings.2c00139.

Oxidizing methionine to map a prion's surface and reveal conformational differences - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L. 2022. Oxidizing methionine to map a prion's surface and reveal conformational differences [Abstract]. Abstracts of the American Chemical Society. Spring 2022 Meeting. March 23, 2022. Abstract number: 365591. https://doi.org/10.1021/scimeetings.2c00113.

Use of methionine oxidation to study prion structure - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L. 2021. Use of methionine oxidation to study prion structure[abstract]. Citation (ID) number 308479, November 5, 2021, Proceedings of the 69th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA and online, October 31-November 4, 2021. Available: https://www.asms.org/docs/default-source/default-document-library/tues-nov-16-12pm---para. llel-2_guide.pdf?.

Cellular prion protein mediates a-synuclein uptake, localization, and toxicity in vitro and in vivo - (Peer Reviewed Journal)
Thom, T., Schmitz, M., Fischer, A., Correia, A., Correia, S., Llorens, F., Pique, A., Mobius, W., Domingues, R., Zafar, S., Stoops, E., Silva, C.J., Fischer, A., Outeiro, T.F., Zerr, I. 2021. Cellular prion protein mediates a-synuclein uptake, localization, and toxicity in vitro and in vivo. Movement Disorders. 37(1):39-51. https://doi.org/10.1002/mds.28774.

Using mass spectrometry to detect the presence of and dissect the structure of prions - (Abstract Only)
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Requena, J.R. 2021. Using mass spectrometry to detect the presence of and dissect the structure of prions. Meeting Abstract. Abstract ID: 355344. https://acs.digitellinc.com/acs/live/8/page/18/1?eventSearchInput=3555344+&eventSearchTrack%5B%5D=23.

Time of detection of prions in the brain by nanoscale liquid chromatography coupled to tandem mass spectrometry is comparable to animal bioassay - (Peer Reviewed Journal)
Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Requena, J.R. 2021. Time of detection of prions in the brain by nanoscale liquid chromatography coupled to tandem mass spectrometry is comparable to animal bioassay. Journal of Agricultural and Food Chemistry. 69(7):2279-2286. https://doi.org/10.1021/acs.jafc.0c06241.

TOR coordinates nucleotide availability with ribosome biogenesis in plants - (Peer Reviewed Journal)
Busche, M., Scarpin, R.M., Hnasko, R.M., Brunkard, J.O. 2021. TOR coordinates nucleotide availability with ribosome biogenesis in plants. The Plant Cell. 33(5):1615-1632. https://doi.org/10.1093/plcell/koab043.

Using targeted mass spectrometry, covalent modification, Western blot, and bioassay to quantitate the relative role of specific lysines in prion replication - (Abstract Only)
C.J. Silva, M.L. Erickson-Beltran, I.C. Dynin. 2020. ANYL 10: Using targeted mass spectrometry, covalent modification, Western blot, and bioassay to quantitate the relative role of specific lysines in prion replication. American Chemical Society Abstracts. ACS Fall 2020 Virtual Meeting & Exposition: 10-ANYL.

Using mass spectrometry to quantify prion polymorphisms and phenotypes in heterozygous sheep and deer - (Abstract Only)
C.J. Silva, M.L. Erickson-Beltran, C. Duque-Velásquez, J.M. Aiken, D. Mckenzie, I. Martín-Burriel, J. Badiola, J.R. Requena, B. Marín, R. Bolea. 2020. AGFD 138: Using mass spectrometry to quantify prion polymorphisms and phenotypes in heterozygous sheep and deer. Abstracts of Papers of the American Chemical Society. ACS Fall 2020 Virtual Meeting & Exposition: 138-AGFD.

A general mass spectrometry-based method of quantitating prion polymorphisms from heterozygous chronic wasting disease-infected cervids - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L., Duque Velásquez, C., Aiken, J.M., McKenzie, D. 2019. A general mass spectrometry-based method of quantitating prion polymorphisms from heterozygous chronic wasting disease-infected cervids. Analytical Chemistry. 92(1):1276-1284. https://doi.org/10.1021/acs.analchem.9b04449.

Quantitating prion polymorphisms from heterozygous CWD-infected white-tailed deer - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Duque Velásquez, C., Aiken, J.M., Mckenzie, D. 2019.Quantitating prion polymorphisms from heterozygous CWD-infected white-tailed deer [abstract]. 8th Iberian Congress on Prions. 8:33-33. ISBN: 978-972-579-050-2. October, 2019.

A general mass spectrometry-based method of quantitating the oxidation of the methionine side chains in classical and atypical scrapie PrPSc - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Martin-Burriel, I., Badiola, J.J., Requena, J.R., Bolea, R. 2019. 238: A general mass spectrometry-based method of quantitating the oxidation of the methionine side chains in classical and atypical scrapie PrPSc [abstract]. Prion. 13(Supplement):131-132.

A general mass spectrometry-based method of quantitating the relative amounts of PrP polymorphisms in CWD PrPSc - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Duque Velasquez, C., Aiken, J.M., Mckenzie, D. 2019. 237: A general mass spectrometry-based method of quantitating the relative amounts of PrP polymorphisms in CWD PrPSc [abstract]. Prion. 13(supplement):131-131.

Distinguishing among protein conformations (prion strains) by detecting covalent differences using quantitative mass spectrometry - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L. 2019. Distinguishing among protein conformations (prion strains) by detecting covalent differences using quantitative mass spectrometry [abstract]. ASM Microbe 2019, June 20-24, 2019, San Francisco, CA. Poster MBP-19.

Exploring the conformational differences between PrPSc from classical and atypical scrapie using mass spectrometry - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Martin-Burriel, I., Badiola, J., Requena, J.R., Bolea, R. 2018. Exploring the conformational differences between PrPSc from classical and atypical scrapie using mass spectrometry. Meeting Abstract. 38(1):65-80. https://doi.org/10.1051/vetres:2006046.

Chronic Wasting Disease: The American experience - (Abstract Only)
Silva, C.J. 2018. Chronic Wasting Disease: The American experience. Meeting Abstract. [abstract]. University of Santiago de Compostela, Spain.