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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Research Project #434474

Research Project: Refining the Relationship between Host Range and Genotype for Macrophomina phaseolina and Optimization of Soil Quantification Assays

Location: Crop Improvement and Protection Research

Project Number: 2038-22000-016-23-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Feb 1, 2018
End Date: Jan 31, 2019

Objective:
The objective of the project is to evaluate the pathogenicity/virulence of isolates of Macrophomina phaseolina on strawberry, examine the population structure of the pathogen, and develop techniques for soil quantification for this pathogen and Fusarium oxysporum f. sp. fragariae.

Approach:
The collaborator at University of California (UC) Cooperative Extension will collect isolates of the pathogen from field samples and evaluate them for pathogenicity on strawberry and a series of other hosts. Virulence tests will also be performed to confirm if there is any host specificity for the isolates. We will conduct simple sequence repeat analysis of the populations and compare California isolates with the population structure found on other hosts in the U.S. (done in collaboration with ARS, Dawson, Georgia). Soil quantification will be examined by evaluating traditional soil plating assays (UC Cooperative Extension) and real time polymerase chain reaction (PCR) methods (ARS Salinas).