Location: Crop Improvement and Protection Research
Project Number: 2038-22000-016-23-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Feb 1, 2018
End Date: Jan 31, 2019
The objective of the project is to evaluate the pathogenicity/virulence of isolates of Macrophomina phaseolina on strawberry, examine the population structure of the pathogen, and develop techniques for soil quantification for this pathogen and Fusarium oxysporum f. sp. fragariae.
The collaborator at University of California (UC) Cooperative Extension will collect isolates of the pathogen from field samples and evaluate them for pathogenicity on strawberry and a series of other hosts. Virulence tests will also be performed to confirm if there is any host specificity for the isolates. We will conduct simple sequence repeat analysis of the populations and compare California isolates with the population structure found on other hosts in the U.S. (done in collaboration with ARS, Dawson, Georgia). Soil quantification will be examined by evaluating traditional soil plating assays (UC Cooperative Extension) and real time polymerase chain reaction (PCR) methods (ARS Salinas).