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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Citrus and Other Subtropical Products Research » Research » Research Project #434437

Research Project: Accelerating Implementation of Huanglongbing (HLB) Tolerant Hybrids - University of California

Location: Citrus and Other Subtropical Products Research

Project Number: 6034-41430-007-06-A
Project Type: Cooperative Agreement

Start Date: Feb 1, 2018
End Date: Dec 14, 2022

Objective:
Determine Huanglongbing (HLB) resistant/tolerant genes in citrus using qualitative trait loci mapping and validate resistance/tolerant markers especially in manderin- and Poncirus-derived plantings. Identify field HLB-resistant/tolerant hybrids in the University of California Breeding program, from selection growing in Florida with help of ARS, and select promising candidates for evaluation of flavor quality by USDA-ARS.

Approach:
Conduct genotyping-by-seqencing and genotyping with single nucleotide polymorphism (SNP) markers in parents and hybrids focusing on mandarin- and Poncirus-derived hybrids. Conduct pNOME approach (sequencing pooled samples of tolerant and susceptible genotypes). Phenotype UCR breeding program citrus lines grown in Florida with help of ARS by determining tree growth, health index, pathogen bacterial titer and disease symptoms.