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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Research Project #434424

Research Project: Developing APCR-free Library

Location: Vegetable Research

Project Number: 6080-22000-028-01-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: May 1, 2018
End Date: Jul 31, 2019

Our objective is to use the latest in sequencing technologies to identify, map and transfer host resistance genes from both wild watermelon (Citrullus amarus) and melon (Cucumis melo) into cultivars suitable for use both domestically and internationally. Resequencing of all available wild watermelon accessions [~130 Plant Introductions (PI)] from the USDA, ARS germplasm repositories, as well as USVL252, USVL114, USVL019. Additionally, we will perform full genome sequencing and assembly of the USDA, ARS multi-resistant melon line MR-1 and the cultivar Ananas Yokneum.

We have isolated DNA from the above listed lines and PI. These lines have been carried forward for a minimum of two generations from the plants of which DNA was isolated by way of hand self-pollinations. These selected and sequenced lines will be maintained by the USDA, ARS group at the United States Vegetable Laboratory (USVL) for use in the ongoing assays for search of host resistance genes. Resequencing data will be used in conjunction with the diseases assays and the Genome-wide association studies (GWAS) to identify genetic regions of resistant lines that are linked to the resistance phenotype. The melon work will be used to generate a high-density genetic map, with full annotation of the multi-resistant line MR-1 and cultivar Ananas Yokneum for use by the research and breeding community.